{"id":"ginkgo-cloud-lab","name":"ginkgo-cloud-lab","summary":"Ginkgo Bioworks Cloud Lab(cloud.ginkgo.bio)でプロトコルを提出・管理してください。","body":"# Ginkgo Cloud Lab\n\n## Overview\n\nGinkgo Cloud Lab (https://cloud.ginkgo.bio) provides remote access to Ginkgo Bioworks' autonomous lab infrastructure. Protocols are executed on Reconfigurable Automation Carts (RACs) -- modular units with robotic arms, maglev sample transport, and industrial-grade software spanning 70+ instruments.\n\nThe platform also includes **EstiMate**, an AI agent that accepts human-language protocol descriptions and returns feasibility assessments and pricing for custom workflows beyond the listed protocols.\n\nThe catalog is organized into **Expression & Purification** (in vitro / cell-free / E. coli / Pichia), **Characterization & Assay**, **Method & Target Onboarding**, and **Specialty**. Pick a protocol below, then read its reference file for inputs, outputs, the automated workflow, and ordering details.\n\n## Available Protocols\n\n### Expression & Purification - In vitro\n\n| Protocol | Readout | Price | Turnaround | Status |\n|---|---|---|---|---|\n| [IVT mRNA/circRNA Synthesis](references/ivt-rna-synthesis-qpcr.md) | qPCR (mRNA or circRNA, 384-well) | $99/sample | up to 12 business days | Certified |\n\n### Expression & Purification - Cell-free (E. coli CFPS)\n\n| Protocol | Readout | Price | Turnaround | Status |\n|---|---|---|---|---|\n| [Validate sequence expression](references/cell-free-protein-expression-validation.md) | Go/no-go titer + purity (up to 1800 bp) | $39/sample | up to 10 days | Certified |\n| [Optimize expression conditions](references/cell-free-protein-expression-optimization.md) | DoE across 24 conditions | $199/sample | up to 11 days | Certified |\n| [Express + quantify (HiBiT)](references/cell-free-protein-expression-hibit.md) | Luminescence, no purification | $39/sample | up to 11 days | Certified |\n| [Express + purify (A280)](references/cfps-strep-tag-purification-a280.md) | Strep-tag, A280 yield | $149/sample | up to 11 days | Certified |\n| [Express + purify minibinder](references/minibinder-strep-tag-a280.md) | Strep-tag, A280, LabChip | $149/sample | up to 11 days | Certified |\n| [Express + purify (A280 + LabChip)](references/cfps-expression-purification-quantification.md) | Strep-tag, A280 + purity/size | $159/sample | up to 12 days | Certified |\n\n### Expression & Purification - E. coli\n\n| Protocol | Readout | Price | Turnaround | Status |\n|---|---|---|---|---|\n| [Express + quantify (HiBiT)](references/ecoli-protein-expression-hibit.md) | Luminescence (up to 384 constructs) | $79/sample | up to 3 weeks | Certified |\n| [Express + purify (A280)](references/ecoli-protein-expression-histag-a280.md) | His-tag, A280 yield | $199/sample | up to 3 weeks | Certified |\n| [Express + purify minibinder](references/ecoli-minibinder-expression-histag-a280.md) | His-tag, A280 yield | $199/sample | up to 3 weeks | Certified |\n| [Express + purify (A280 + LabChip)](references/ecoli-expression-purification-quantification.md) | His-tag, A280 + purity/size | $209/sample | up to 3 weeks | Certified |\n\n### Expression & Purification - Pichia\n\n| Protocol | Readout | Price | Turnaround | Status |\n|---|---|---|---|---|\n| [Express + quantify (LabChip)](references/pichia-protein-expression-labchip.md) | Secreted protein, size/purity (up to 96) | $89/sample | up to 4 weeks | Certified (New) |\n\n### Characterization & Assay\n\n| Protocol | Readout | Price | Turnaround | Status |\n|---|---|---|---|---|\n| [Express + thermal shift](references/cfps-strep-purification-thermal-shift.md) | SYPRO Orange Tm (Tonset, TM1-3) | $159/sample | up to 12 days | Certified |\n| [Detect enzymatic products (Echo-MS)](references/echo-ms-cfps-detection.md) | Substrate/product by Echo-MS | $44/sample | up to 13 days | Beta |\n\n### Method & Target Onboarding\n\n| Protocol | Readout | Price | Turnaround | Status |\n|---|---|---|---|---|\n| [Onboard Echo-MS method](references/echo-ms-method-onboarding.md) | Calibration curve, LOD/LOQ | $799/molecule | up to 3 weeks | Certified |\n| [Onboard SPR target](references/spr-target-onboarding.md) | Validated SPR capture method | $1,399/target | up to 4 weeks | Beta |\n\n### Specialty\n\n| Protocol | Readout | Price | Turnaround | Status |\n|---|---|---|---|---|\n| [Generate fluorescent pixel art](references/fluorescent-pixel-art-generation.md) | UV photo, 7-color E. coli palette | $25/plate | up to 7 days | Beta |\n\n**Coming soon:** Protein Expression and Binding Affinity Characterization (express + purify, then screen binding affinity against a target).\n\n## Choosing a Protocol\n\n- **Quick expressibility screen?** Cell-free HiBiT ($39) or Validate sequence expression ($39).\n- **Need purified protein + yield?** A280 tiers (cell-free or E. coli); add LabChip for purity/size.\n- **Difficult / membrane / disulfide / cofactor targets?** Cell-free Optimize (24-condition DoE).\n- **Secreted or eukaryotic targets?** Pichia expression.\n- **Screening de novo binders/minibinders?** Cell-free or E. coli minibinder tiers, then SPR onboarding for kinetics.\n- **Enzyme activity / biocatalysis?** Echo-MS enzymatic detection (onboard the analyte method first).\n- **Stability / developability ranking?** Thermal shift assay.\n- **RNA (mRNA/circRNA)?** IVT synthesis + qPCR.\n\n## General Ordering Workflow\n\n1. Select a protocol at https://cloud.ginkgo.bio/protocols\n2. Configure parameters (number of proteins/samples/molecules/targets, replicates, plates)\n3. Download the protocol's input template and upload inputs (FASTA/CSV/XLSX for sequence protocols; Design Tool for pixel art; vendor catalog numbers for onboarding)\n4. Add any special requirements in the Additional Details field\n5. Provide an email, agree to the protocol terms, and add to cart / submit to receive a feasibility report and price quote\n\nFor protocols not listed above, use the **EstiMate** chat (https://cloud.ginkgo.bio/estimate) to describe a custom protocol in plain language and receive a compatibility assessment and pricing.\n\n## Authentication\n\nAccess Ginkgo Cloud Lab at https://cloud.ginkgo.bio. Account creation or institutional access may be required. Contact Ginkgo at cloud@ginkgo.bio for access questions.\n\n## Key Infrastructure\n\n- **RACs (Reconfigurable Automation Carts):** Modular robotic units with high-precision arms and maglev transport\n- **Catalyst Software:** Protocol orchestration, scheduling, parameterization, and real-time monitoring\n- **70+ integrated instruments:** Agilent Bravo liquid handlers, Beckman/Labcyte Echo acoustic dispensers, BMG PHERAstar / Tecan Spark readers, Revvity LabChip, Bio-Rad CFX Opus, Nicoya Alto SPR, SciEx Echo-MS, Inheco/Cytomat incubators, and more\n- **Nebula:** Ginkgo's autonomous lab facility in Boston, MA","author":"@K-Dense-AI","ownerProfile":null,"authorContacts":null,"sourceUrl":"https://github.com/K-Dense-AI/scientific-agent-skills/tree/main/skills/ginkgo-cloud-lab","license":"MIT","category":"productivity","lang":"en","tokens":1706,"stars":0,"calls30d":1,"claimed":false,"visibility":"public","origin":"crawler","version":"0.1.0","createdAt":"2026-08-22","updatedAt":"2026-08-22","files":[{"path":"references/cell-free-protein-expression-hibit.md","size":2105,"sha256":"c93d29199800737a37bd2d5e13af7b7dbeaf049864f8990cdc11471394db88e6"},{"path":"references/cell-free-protein-expression-optimization.md","size":2657,"sha256":"a55de455e4bc0ec6a79d1df002baad0018c905e299180fbb9d699a53ed11538c"},{"path":"references/cell-free-protein-expression-validation.md","size":2018,"sha256":"0af3fe33052fe8bcb2de62d05f38870c3c7e96ff9d1be0f04161c0e425dee896"},{"path":"references/cfps-expression-purification-quantification.md","size":2457,"sha256":"36299ce12b5d8805aee78377fd5aa40f1f667c0947bf57122b6ce8c2f7582fd4"},{"path":"references/cfps-strep-purification-thermal-shift.md","size":3180,"sha256":"9f694714e0f24546e7e0ca11fd2bca2100de42c871413feaa2577a58acc2ec3f"},{"path":"references/cfps-strep-tag-purification-a280.md","size":2226,"sha256":"9f57114e902d57527dcca1f50cd535d24d79ebc2e974b417e487f143e824d4a1"},{"path":"references/echo-ms-cfps-detection.md","size":1856,"sha256":"8559bd9cc4b42282b65a3cf112654797948824ddde89fdf8ead61a88356ef47b"},{"path":"references/echo-ms-method-onboarding.md","size":2429,"sha256":"d2fe8ae43474fbbd4f16bcc0bd69210d27a649b0ef2a38e490acd81b48673f86"},{"path":"references/ecoli-expression-purification-quantification.md","size":2362,"sha256":"7ba569df772167a1fc2cdf3c8f01a692e7e2daa7749d37078036ee6b2166579a"},{"path":"references/ecoli-minibinder-expression-histag-a280.md","size":2494,"sha256":"08353f78bdb475f1b903f51dd075b9ce8681e56661e3b219af2321d331b3130a"},{"path":"references/ecoli-protein-expression-hibit.md","size":1975,"sha256":"feb6c584536a460deea218ec30e3518ac3a10f4d7f59250dd87add91ec4d44eb"},{"path":"references/ecoli-protein-expression-histag-a280.md","size":2127,"sha256":"89d1214a869befde38d172cb364d6088646cebfb649c2c88552a31e7bd42e846"},{"path":"references/fluorescent-pixel-art-generation.md","size":2893,"sha256":"19978904607739dde30af0c6c2b0465018e8b11d3872f246621223d130098a53"},{"path":"references/ivt-rna-synthesis-qpcr.md","size":2170,"sha256":"3b955bef645452fe9656f7f01d700e746f73c14e920eff94626d821d73b274a6"},{"path":"references/minibinder-strep-tag-a280.md","size":2272,"sha256":"7552088b8502d4793752d94cb2c8401ffaae09db03af6a47503c38616c3beda7"},{"path":"references/pichia-protein-expression-labchip.md","size":2045,"sha256":"2ba279ce0dc6dc6237f0f4f71bf90cfa81742bd9b3a7e4084881ff6fcd864f9d"},{"path":"references/spr-target-onboarding.md","size":2074,"sha256":"1024b20128ee8ac884270f4cd24b867ec00f5082741f0123381cc0a1b2c72d51"}],"requires":{"mcp":[],"tools":["Read"]},"safety":{"flags":[],"scannedAt":"2026-08-22","hasScripts":false,"networkEndpoints":["cloud.ginkgo.bio"]}}